World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Chronic Levodopa Administration Followed by a Washout Period Increased Number and Induced Phenotypic Changes in Striatal Dopaminergic Cells in Mptp-monkeys, Volume 7

By Mosley, R., Lee

Click here to view

Book Id: WPLBN0003935073
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Chronic Levodopa Administration Followed by a Washout Period Increased Number and Induced Phenotypic Changes in Striatal Dopaminergic Cells in Mptp-monkeys, Volume 7  
Author: Mosley, R., Lee
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Description
Description : In addition to the medium spiny neurons the mammalian striatum contains a small population of GABAergic interneurons that are immunoreactive for tyrosine hydroxylase (TH), which dramatically increases after lesions to the nigrostriatal pathway and striatal delivery of neurotrophic factors. The regulatory effect of levodopa (L-Dopa) on the number and phenotype of these cells is less well understood. Eleven macaques (Macaca fascicularis) were included. Group I (n = 4) received 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP) and L-Dopa: Group II (n = 4) was treated with MPTP plus vehicle and Group III (n = 3) consist of intact animals (control group). L-Dopa and vehicle were given for 1 year and animals sacrificed 6 months later. Immunohistochemistry against TH was used to identify striatal and nigral dopaminergic cells. Double and triple labeling immunofluorescence was performed to detect the neurochemical characteristics of the striatal TH-ir cells using antibodies against : TH, anti-glutamate decarboxylase (GAD67) anti-calretinin (CR) anti-dopa decarboxylase (DDC) and anti-dopamine and cyclic AMP-regulated phosphoprotein (DARPP-32). The greatest density of TH-ir striatal cells was detected in the striatum of the L-Dopa treated monkeys and particularly in its associative territory. None of the striatal TH-ir cell expressed DARPP-32 indicating they are interneurons. The percentages of TH-ir cells that expressed GAD67 and DDC was approximately 50%. Interestingly, we found that in the L-Dopa group the number of TH/CR expressing cells was significantly reduced. We conclude that chronic L-Dopa administration produced a long-lasting increase in the number of TH-ir cells, even after a washout period of 6 months. L-Dopa also modified the phenotype of these cells with a significant reduction of the TH/CR phenotype in favor of an increased number of TH/GAD cells that do not express CR. We suggest that the increased number of striatal TH-ir cells might be involved in the development of aberrant striatal circuits and the appearance of L-Dopa induced dyskinesias.

 

Click To View

Additional Books


  • Plos One : Potentials-attract or Likes-a... (by )
  • Plos One : T8;9P22;P24, Volume 7 (by )
  • Plos One : Correlation of Gene Expressio... (by )
  • Plos One : Estimating the Impact of Stat... (by )
  • Plos One : Validation of the ‘‘world Hea... (by )
  • Plos One : Diuretics Prime Plant Immunit... (by )
  • Plos One : Inhibition of Proliferation a... (by )
  • Plos One : Granzyme B-induced Neurotoxic... (by )
  • Plos One : Heat Shock Protein 70 Express... (by )
  • Plos One : a Molecular Phylogeny for Ypo... (by )
  • Plos One : Prevalence and Associated Ris... (by )
  • Plos One : New Insights on the Sialidase... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Fair are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.